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An infrared spectrophotometer is an instrument that passes infrared light through an organic molecule and produces a spectrum that contains a plot of the IR spectrum gives sufficient information about the structure. (identification of functional groups) of a compound and can also be used as analytical tool to. Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample. Factors affecting vibrational Frequency. • Instrumentation. • Sampling Techniques. • IR Spectrum-Examples. • Advantages & disadvantages. • Applications Detecting instrument. Visual (most common) representation, or. Spectrum. SPECTROSCOPY - Study of spectral information. Upon irradiation with infrared light, absorption of IR radiation is related to the changes of (mid infrared spectroscopy, Mid IR, MIR). 12 500 – 4 000 Instrumentation for IR spectroscopy.SOURCE OF IR RADIATION. The most important source of infra red light for scanning the spectrum of an organic compound is NERNST GLOWER consisting. IR Spectroscopy detects frequencies of infrared light that are absorbed by a molecule. Molecules tend to absorb these specific frequencies of light since they The instrumentation of infrared spectroscopy is illustrated below. First, a beam of IR light from the source is split into two and passed through the reference Infrared spectroscopy. Instrumentation FT-IR spectrometer has significant advantages over dispersive one Transmission IR spectroscopy (TIRS).