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HPLC. High pressure liquid chromatography (HPLC) is a method for separating substance mixtures, determining substances and measuring their concentration. The Waters 515 HPLC Pump is for research use only. Calibrating In the stand-alone mode, the 515 Pump manually controls isocratic analyses. Precautions are outlined in “CDC Guidelines on. Specimen Handling,” CDC – NIH Manual, 1984. Calibration. Follow acceptable methods of calibration with pure This operator's manual contains information needed to install, operate, and perform user maintenance on the Series 1500 Digital HPLC Pump.Use the Waters 1525, 1525µ, and 1525EF HPLC pumps and the optional. 1500-Series Column Heater Connecting the inject start signal (for manual injector). AZURA® Pump P 2.1S/P 4.1S Instructions V6870. 1. 1.1 Device overview. The HPLC pumps P 4.1S/ P 2.1S with pump heads can be used as feed. After mixing, the next component in the hydraulic circuit is the purge valve, which is used to manually direct the mobile phase towards the outlet filter or