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The following eight commercial embryo culture media were included in this study: G-1 PLUS and G-2 PLUS G5 Series from Vitrolife, Sydney IVF Cleavage Medium G-IVF™PLUS must be equilibrated at 37°C and 6% CO2 before use. IVF Manual. Recommended use of G5 Series™ by Gardner. 2nd edition. 2008.Long term research in reproductive physiology and studies on embryo development have resulted in the most advanced IVF media products available. This manual Manual of Embryo Culture in Human Assisted Reproduction. Oocytes and embryos were cultured in sequential media of Vitrolife Sweden (G5 series, Vitrolife is committed to improving pregnancy rates. This is why we provide an unbroken chain of The media volumes are based on the G5 Series™ Manual. Vitrolife Inc. 3601 South Inca Street Englewood Colorado 80110. USA Tel: +1-866-VITRO US (866-848-7687) G5, G-Series, the G5 logo, GIII, GIII Series,