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vector according to the EasySelect Pichia Expression Kit Manual (Invitrogen) and Atherosclerotic Plaque, Pichia pastoris and Single-Chain Antibodies Easyselect Pichia Expression Kit Manual, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. EasySelect™ Pichia Expression Kit. A Manual of Methods for Expression of Recombinant. Proteins Using pPICZ and pPICZα in Pichia pastoris. Catalog no. Refer to the Pichia Expression Kit manual for details. Once your protein is expressed, follow the protocol below to prepare a cell lysate for chromatography on separately transformed into P. pastoris GS115 cells by electroporation according to the instructions of the Pichia Expression Kit Manual (Invitrogen).