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bcl2fastq v1.8.4, and refer to the bcl2fastq Conversion User Guide Version v1.8.4 for instructions. BCL Conversion/Demultiplexing Directory Structure. to convert BCL to FASTQ, install bcl2fastq v1.8.4, and refer to the bcl2fastq Conversion User Guide Version v1.8.4 (part # 15038058) for instructions. bcl2fastq performs Bcl conversion and demultiplexing in a single step, and puts the resulting demultiplexed compressed FASTQ files in /Data/ bcl2fastq Conversion Software v1.8.4 User Guide. Instructions for using the bcl2fastq Conversion Software v1.8.4 to demultiplex data and convert BCL files. March 2013. bcl2fastqConversion. User Guide. Version 1.8.4. FORRESEARCH USEONLY. Introduction. 3. Installing bcl2fastq. 8. Bcl Conversion Input Files. Demultiplexing Chromium data with Illumina bcl2fastq requires the correct you should be able to follow the pipeline instructions, with one caveat. bcl2fastq Conversion Software v1.8.4 User Guide (15038058 B) | Manualzz section describes how to perform Bcl conversion and demultiplexing in bcl2fastq.name: bcl2fastq; version: 2.19.1. description: Demultiplexes data and converts BCL files generated by Illumina sequencing systems to standard FASTQ file The instructions in this document must be strictly and explicitly followed by Download the bcl2fastq2 Conversion Software v2.20 from the bcl2fastq