Overview of the global situation of African swine fever since 2016 and bi-weekly updates of African swine fever events based on the reports submitted to the OIE. Asia and the Pacific. A weekly summary of African swine fever events submitted to the OIE by countries in the region since 2018. 1/2. The Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Chapter 1.1.6. - Principles and methods of validation of diagnostic assays for infectious diseases and Section 2.2. - Validation of diagnostic tests), as well as the requirements of the ISO/IEC 17025 provide guidance for validating diagnostic methods to ensure they are Laboratory tests are essential for the diagnosis of African swine fever (ASF) and are key to the success of ASF surveillance activities. Chapter 3.9.1. of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Terrestrial Manual) describes the recognised international standards for ASF diagnosis. However, in certain circumstances, the timely submission, processing and testing This manual provides a guide for cross-border qualitative risk assessment as performed during the ASF Cross-Border Risk Assessment - South-East Asia project. The objective is to provide detailed guidance for performing a risk assessment for the entry of ASFV into a country. The manual is based on the OIE Terrestrial Animal Health Code Chapter 2 African swine fever (ASF) is a severe viral disease affecting domestic and wild pigs. In domestic pigs it is responsible for serious production and economic losses, primarily due to high mortality. This transboundary animal disease (TAD) can be spread by live or dead pigs, domestic or wild, and pork products.. Furthermore, transmission can also occur via contaminated feed and fomites (non It is the first African swine fever (ASF) kit to be registered by the OIE. "The addition of our ASF detection kit to the OIE manual marks a key milestone in our effort to help our customers address the epidemic," said Peter Vuong, General Manager AgriBusiness. "Veterinary authorities and labs now have access to a validated diagnostic tool In this scenario, efficient risk communication becomes an essential component of the disease response. Yet communication challenges exist. Given that ASF does not pose a direct threat to human health, risk perception among some of the key actors continues to be low. Communication efforts must highlight the impact of the disease on animal health • Standing Group of Experts on ASF for Asia • OIE Terrestrial Animal Health Code -Establishing a country, zone & compartment free of ASF -Recommendations relating to trade of pork & pork products • OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals • ASF-related webinars • Other resources are available online at OIE Procedure for the extraction of the ASF virus (ASFV) DNA. SOP/CISA/ASF/DNA EXTRACTION/1/ Method accredited UNE-EN ISO/IEC 17025:2017. Detection of the ASFV genome by the OIE-conventional PCR, Agüero et al., 2003. SOP/CISA/ASF/PCR/1 Method accredited UNE-EN ISO/IEC 17025:2017. Detection of the ASFV genome by the OIE- real time PCR (1), King et The OIE is organising a regional ASF cross-border risk assessment study in South-East Asia with technical support from Hong Kong City University. As part of the study, a serial of webinars and exercises will be organised to cover different topics of ASF risk assessment. 1. An overview of African swine fever epidemiology (Prof. Dirk Pfeiffer ) 2. OIE international standards . ASF